Open topic with navigation

Adding Plates

In this tutorial, we explain how to properly add or load a plate to Rock Imager, and present the options for what you can do after you load the plate(s).

Prerequisites

• You have created an experiment in Rock Maker. If you aren't interested in tracking conditions with Rock Maker, you can create a proxy experiment to expedite the imaging process.
• Your plate has a barcode label affixed to the short side of the plate opposite the A1 position.

To add a plate to Rock Imager:

1. Open the door of the load port.
2. Place plates into the load port. Plates should be inserted with the barcode facing away from you. Plates can be placed in any slot, in any order.
3. Close the load port door. Make sure to turn the handle to lock the door for optimal temperature control.
4. Choose from the following options: 
   
   • If you have Rock Imager 1000 or 182, you can move the plate to storage. Moving plates to storage is recommended if you are using Rock Imager 1000 because of greater temperature control. When you move a plate to storage, Rock Imager takes the plate and puts it in a storage hotel away from the load port. The imager will then image the plate according to its automatic imaging schedule.
     
     To move the plate(s) in the load port to storage, on the Load Port menu, click Store Contents. Rock Imager will automatically scan the contents of the load port, and move the plate(s) to storage.
   • If you want to manually inspect a plate, first you must tell the imager to scan the barcode: On the Load Port menu, click Scan Load Port. Locate the Load Port sub-tab (found on the Plates tab) at the bottom-left of your screen. Right-click on the plate of interest and select Move Plate To, and then select the imaging method you want to use (e.g. Visible, UV, etc.).
   • If you want Rock Imager to image a plate immediately so you can view, score, and annotate images in Rock Maker, first scan the barcode (on the Load Port menu, click Scan Load Port), then locate your plate on the Load Port sub-tab of the Plates tab. Right-click on the plate and select Image Plate (default imaging setting) or Image Plate Using (custom imaging setting). Note: Rock Imager Processor must be running in order for Rock Maker to receive images.
   
   

Related Topics

• Initiating an Imaging Session
• Manually Inspecting Plates
• Viewing a Plate's Schedule
• Creating a Proxy Experiment in Rock Maker
• Automatic Mode
• Plates Tab
• Load Port Sub-Tab

RIC-V216R216	10 DeAngelo Drive Bedford, MA 01730 USA   +1-781-788-0228 support@formulatrix.com publications@formulatrix.com